【文献标题】Dietary prebiotic inulin benefits on growth performance, antioxidant capacity, immune response and intestinal microbiota in Pacific white shrimp (Litopenaeus vannamei) at low salinity

【作者】Li Zhou，Huifeng Li，Jian G. Qin，et.al

【作者单位】海南大学（Hainan University）

【文献中引用产品】

虾蛋白酶（Protease）ELSIA试剂盒

虾淀粉酶（Amylase）ELISA试剂盒

虾脂肪酶（Lipase）ELISA试剂盒

【关键词】Low salinity，Inulin，Growth performance，Antioxidant capacity，Intestinal microbiota，Litopenaeus vannamei

【DOI】doi.org/10.1016/j.aquaculture.2019.734847

【影响因子(IF)】3.42

【出版期刊】《Aquaculture》

【产品原文引用】

2.7. Biochemical assays

The entire intestinal of three shrimps mixed as one sample, the hepatopancreas of one shrimp as one sample, each group have six samples. All samples were weighed and homogenized with 9 vol (v/w)of 0.86% physiological saline. Then, the homogenate was centrifuged at 2500 ×g at 4 °C for 10 min and the supernatant was collected for biochemical assays according to the manufacturer's instruction.Activities of intestinal protease, amylase, and lipase were measured using an enzyme-linked immune sorbent assay (ELISA) kit (Shanghai Hengyuan Biotechnology Co, Ltd) (Li et al., 2019). Phenol oxidase (PO)activity in hepatopancreas was measured using an enzyme-linked immune sorbent assay (ELISA) kit (Nanjing Jiancheng Institute, China).The contents of malondialdehyde (MDA), total superoxide dismutase (T-SOD), catalase (CAT), acid phosphatase (ACP) in hepatopancreas were measured by using specific commercial assay kits (Nanjing Jiancheng Institute, China) (Zhang et al., 2008). Results were recorded on a microplate reader (Epoch, BioTek, USA).Total SOD activity was determined by using WST-1 method. One unit of SOD activity was defined as the amount of enzyme required for 1 mg tissue proteins in 1 mL of a reaction mixture SOD inhibition rates to 50% as monitored at 550 nm.CAT activity was determined by using ammonium molybdate colorimetric method. One unit of CAT activity was defined as 1 mg tissue proteins consumed 1 μmol H2O2 at 405 nm for 60 s. MDA content was determined by using TBA method, which was measured at 532 nm. ACP activity was determined by using colorimetric method. One unit of ACP activity was defined as 1 mg tissue proteins produced 1 mg phenol at 37 °C for 30 min as monitored at 550 nm. Activities of protease, amylase, lipase and phenol oxidase were measured using the enzyme-linked immunosorbent assay (ELISA) and the absorbance (OD) was measured at 450 nm. Total protein content was measured by using Coomassie brilliant blue method.

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