干扰素γ(IFNg)活性蛋白

Active Interferon Gamma (IFNg)

IFN-G; IFG; IFI; INFr; IFN, Immune Interferon

仅供体外研究使用，不用于临床诊断！请索取进口关税税单及报关单！

• 细胞因子

• 感染免疫

• 编号APA049Hu01

• 物种Homo sapiens (Human，人)

• 品牌商.(美国)

• 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）

• 性状冻干粉

• 纯度> 95%

• 等电点9.6

• 应用Cell culture; Activity Assays; In vivo assays.

• 下载英文说明书   中文说明书

• 规格10ug50ug250ug1mg1g

活性实验

IFN-γ is a dimerized soluble cytokine that is the only member of the type II class of interferons. The importance of IFNγ in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. As reported, IFNγ is an important activator of human monocytic THP1 cells. Therefore, THP-1 cells were incubated in RPMI 1640 with various concentration of IFN-γ, then cells were observed by inverted microscope everyday. After stimulated with IFN-γ (2ng/mL) for 5 days, morphological changes occurred in THP1 cells which displayed the shape of fusiform or polygon and were more likely to adhere. Effect of IFN-γ on THP1 cells is shown in Figure 1.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

γ干扰素(IFN-γ)ELISA试剂盒【原理介绍】IFNγ，γ干扰素。和α,β干扰素并无明显同源性。由受同种抗原、肿瘤、和分裂因子刺激的活T细胞NK细胞所分泌。IFNγ具有一系列的生物学功能：抗病毒作用，抑制肿瘤细胞生长，促进B细胞产生抗体。此外，IFNγ活巨噬细胞，增强NK细胞的细胞毒作用，刺激T细胞的细胞毒作用。

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