【文献标题】Physiological-biochemical responses of wheat to blending controlled-release potassium chloride and soluble potassium chloride

【作者】Zeli Li , Weitao Zhang , Lixue Qiu ,et.al

【作者单位】山东农业大学（ Shandong Agricultural University）

【文献中引用产品】

植物生长素（IAA）ELISA试剂盒

植物脱落酸(ABA)ELISA试剂盒

【关键词】Root scanner ，Root characteristics ，Photosynthetic rate ，Endogenous hormone ，Potassium

【DOI】doi.org/10.1016/j.still.2021.105058 

【影响因子(IF)】8.6

【出版期刊】《Soil & Tillage Research》

【产品原文引用】

2.3. Sampling and chemical analyses 

The yields were five consecutive years (2014–2019), so we explained the mechanism of increasing yield of controlled-release potassium chloride through physiological and biochemical indexes during growing seasons 2018? 2019. Samples from undisturbed surface soil (0–20 cm) were collected at seedling stage (31 days after fertilizing), re-greening stage (157 days after fertilizing), and after topdressing at jointing stage (188 days after fertilizing), grain-filling stage (210 days after fertilizing) and at maturity (245 days after fertilizing). At jointing and grain-filling stages, relative chlorophyll content (SPAD value) was determined by a chlorophyll meter (SPAD-502, Minolta, Japan) and conducted by Li et al. (2019). The photosynthesis of the ear leaf was measured by a Li-6400XT portable photosynthesis system (Licor Inc., Lincoln, USA). The leaf area index (LAI) by a canopy analyzer (LAI-2200, Beijing, China) was evaluated at five different points. Leaf and root samples were collected from the wheat during the jointing and grain-filling growth stages. Auxin (IAA) and abscisic acid (ABA) were estimated using the ELISA kit provided by Shanghai Hengyuan Biological 114 Technology Co. Ltd. (Shanghai, China). Soil samples of plowed horizons (0–20 cm) were collected from each plot, then air-dried in the laboratory, and any visible roots, organic residues, and stone fragments were removed manually, used by 2-mm, and 0.25-mm sieves, and further analyses. The available K of soil was extracted with 1.0 mol L? 1 of CH3COONH4 (7.0 pH) and analyzed with a flame photometer (MODEL410, Sherwood, England). 

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