【文献标题】Interactions between phosphorus availability and microbes in a wheat–maize double cropping system: A reduced fertilization scheme
【作者】YU Xiao-jing, CHEN Qi, SHI Wen-cong, et.al
【作者单位】山东农业大学(Shandong Agricultural University)
【文献中引用产品】
土壤酸性磷酸酶(ACP)ELISA试剂盒
土壤碱性磷酸酶(ALP)ELISA试剂盒
【关键词】 wheat–maize rotation, Olsen-P, alkaline phosphatase, phosphorus fertilization, calcareous soils
【DOI】doi.org/10.1016/S2095-3119(20)63599-7
【影响因子(IF)】4.38
【出版期刊】《Journal of Integrative Agriculture》
【产品原文引用】
At the 12-leaf collar stage (V12) of maize in 2018, the following analyses were performed: (i) analysis of acid phosphatase (ACP) and ALP activity in the collected samples of fresh root and rhizosphere soil by using an ELISA Kit (Shanghai HengYuan Biological Technology Co., Ltd., Shanghai, China); (ii) analysis of ADP-glucose pyrophosphorylase (AGpase) of photosynthetic enzymes through enzyme-linked immunosorbent assay in the largest functional leaf samples collected; and (iii) determination of the net photosynthesis (Pn) rate of the largest functional leaf by using the LI-6400XT Portable Photosynthesis System (LI-Cor, Lincoln, NE, USA). After the harvest of each crop, the grain and straw were separated for the analysis of dry weight and total P (TP) content.
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